《植物生理学报》 2015, 51(6): 955-961

CuO纳米颗粒对拟南芥叶片生长及生理特性的影响

徐立娜, 王震宇*, 赵建

中国海洋大学环境科学与工程学院, 山东青岛266100

通信作者：王震宇；E-mail: wang0628@ouc.edu.cn；Tel: 0532-66782092

摘　要：

本文通过营养液培养实验, 研究CuO纳米颗粒(nanoparticles, NPs)悬浮液对拟南芥叶片生长、叶绿体活性氧产生和保护酶活性的影响。结果表明, CuO NPs悬浮液处理96 h后, 拟南芥叶片生长受到显著抑制, 叶绿体中过氧化氢(H₂O₂)、羟基自由基(OH•)和超氧阴离子(O₂–•)含量均随着NPs处理浓度的增加和时间的延长而逐渐上升; 超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)及抗坏血酸过氧化物酶(APX)的活性和丙二醛(MDA)含量增加。这表明CuO NPs能激发植物细胞的防御反应, 这种防御反应与CuO NPs存在时间-剂量关系。

关键词：CuO纳米颗粒; 叶片生长; 叶绿体; 氧化胁迫

收稿：2015-03-19   修定：2015-05-25

资助：国家自然科学基金(41403086、41325013和41120134004)。

Effects of CuO Nanoparticles on Growth and Physiological Characteristics in Leaf of Arabidopsis thaliana

XU Li-Na, WANG Zhen-Yu*, ZHAO Jian

College of Environmental Science and Engineering, OceanUniversity of China, Qingdao, Shandong 266100, China

Corresponding author: WANG Zhen-Yu; E-mail: wang0628@ouc.edu.cn; Tel: 0532-66782092

Abstract:

The purpose of the present study was to estimate the influence of CuO nanoparticles (NPs) suspension on the leaf growth of Arabidopsis thaliana, reactive oxygen generation in chloroplast, and antioxidant enzyme activity under hydroponic conditions. The results showed that the leaf growth was significantly inhibited exposure to CuO NPs suspension for 96 h. The contents of H₂O₂, OH• and O₂ –• increased gradually with the increasing of the time and the concentration of CuO NPs suspension, and the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX) and malonaldehyde (MDA) content increased treated with CuO NPs. It was concluded that CuO NPs could excite the defensive reactions of plant cell, which had the time- and concentration-dependent of CuO NPs exposure.

Key words: CuO nanoparticles; leaf growth; chloroplast; oxidative stress